Analysis of Artificial Sweetners Using the LaChromUltra HPLC

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Aspartame and acesulfame K are artificial sweeteners used to formulate many sugar-free foods and beverages. Replacing sugar with artificial sweeteners reduces caloric intake and dental decay, and provides diabetic patients with additional options to control sugar intake. Since artificial sweeteners are often much sweeter than sugar, it is important to accurately measure their concentrations in food and beverages. Presented here is a method for analysis of aspartame, acesulfame K, and caffeine in a sugar-free carbonated beverage using the Hitachi LaChromUltra HPLC.