Analysis of Chlorogenic Acid in Coffee Using the LaChromUltra HPLC System with UV Detection

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Chlorogenic acid is a biologically important phenolic molecule involved in several biosynthetic pathways, and has been shown to affect the level of glucose in the bloodstream. It is important to know the concentration of this compound in various foodstuffs. Here we describe a method to analyze chlorogenic acid in coffee using the Hitachi LaChromUltra HPLC.